Histochemistry Laboratory

Staff:

• Dr. Małgorzata Żaba, PhD

Laboratory equipment

• ThermoShandon Varistain GEMINI Slide Stainer
• Microm HM355S Microtome with STS Transfer System
• Leica RM 2145 Microtome
• Leica RM 2125 RT Microtome
• Others

Laboratory services

The laboratory performs routine paraffin processing and special stains including PAS, Masson trichrome, van-Gieson, Congo red stain and others.  

Immunohistochemistry Laboratory

Staff:

• Magorzata Żaba, PhD

Laboratory equipment

• Lab VisionTM Autostainer 360-2D with PT Module
• Cryotome FSE^® Thermo Shandon
• Cryotome LEICA CM 1100
• Jouan VXE 490 and VES 490 Freezers
• Others

Laboratory services

The laboratory performs diagnostic and research immunohistochemistry on cryo- and paraffin sections.

Laboratory of Electron Microscopy (TEM/STEM)

Staff:

• Dr Natalia Matysiak, PhD
• Łukasz Mielańczyk, MSc

Laboratory equipment

• Tecnai G² Spirit BioTWIN STEM equiped with TEM tomography, EDS spectroscopy, cryoTEM&Low Dose.
• Vitrobot^TM Mk IV
• Ultramicrotome Leica EM UC7/FC7 with micromanipulator from Diatome
• Ultramicrotome RMC PowerTome PT-X
• Leica EM IGL
• Leica EM AFS2 (Automatic Freeze Substitution-System)
• Leica EM PACT2 (High Pressure Freezer)
• Leica EM KMR3
• Leica EM S4E
• others

Laboratory services

The laboratory performs routine transmission electron microscopy study. In addition, we perform all electron microscopy specimen preparation techniques including cryofixation, freez substitution, high pressure freezing, and immunogold labeling. We have specialized in the cryo-electron microscopy investigations.   

Laboratory of Atomic Force Microscopy

Staff:

• Dr Olesya Klymenko, D.Sc.

Laboratory equipment

• Nikon Eclipse Ti-E Microscope
• Bruker BioScope Catalyst AFM
• Others

Laboratory services

The laboratory is dedicated to study structure of individual molecules. In addition, the AFM laboratory is prepared to monitor biological reactions of living cells.

Laboratory of Digital Holographic Microscopy

Staff:

• Dr Olesya Klymenko, D.Sc.
• Dr Daria Gendosz de Carrillo, PhD

Laboratory equipment

• Nanolive 3D Cell Explorer Holographic Microscope
• Others

Digital holographic microscopy is a type of microscopy that combines digital holography with microscopy to allow transparent cells to be visualized with classic cell culture plates. Digital holographic microscopy is distinct from other types of microscopy because it does not recreate a projected image of the specimen but instead produces a digital hologram from the light waves that have passed by the specimen. A numerical reconstruction algorithm in a computer is used to create the hologram. In traditional microscopy, an imaging forming lens is used to create the image sample.

Nanolive's technology is the unique solution to offer 3D label-free live cell imaging. The extremely low light power that generates the holograms allows for a total absence of phototoxicity which leads to excellent time resolution. Due to this technology we are capable of exploring living cellular processes with real-time kinetics. Therefore, without the need for cell fixation and staining, we are able to obtain native information on single cell characteristics such as mitochondrial and cellular metabolism, cell-cell and cell-material interactions and cytotoxicity.